High-efficiency electro-transformation ofEscherichia coliwith DNA from ligation mixtures
نویسندگان
چکیده
منابع مشابه
High-efficiency transformation of mammalian cells by plasmid DNA.
We describe a simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells. In this protocol, the calcium phosphate-DNA complex is formed gradually in the medium during incubation with cells and precipitates on the cells. The crucial factors for obtaining efficient transformation are the pH (6.95) of the buffer used for th...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.6.1653